Bioingenium is a service company CDMO (Contract Development & Manufacturing Organization) offering Process Development & Custom Manufacturing Services for most types of biologics, covering from early stages of development (Proof-of-concept & Pre-clinical) until industrial scale manufacturing.

With a large experience in upstream and downstream processes, we offer flexible and scalable equipment for producing and purifying therapeutic proteins.

Bioingenium has a proprietary technology for heterologous Protein expression in Pichia pastoris and Bacteria.

We have the knowhow and capabilities to produce your protein of interest from small scale to pilot plant scale, we can develop your production process make it robust and affordable.

Our services include:

- Strain development.

- Production of mg quantities for proof-of-concept in bacteria, Pichia pastoris, CHO and HEK cells.

- Fermentation/cell culture process development.

- Purification process development.

- Formulation of proteins.

- Process scale-up.

- Development of analytical tools.

  • Area of Activity
  • CRO - CMO Services
  • Proteins
  • Manufacture and commercialization of fermentation or biocatalysis
  • Company type
  • Services and consulting for biotech sector
  • Health Biotechnology
  • Industrial Biotechnology - Manufacturers
c/ Baldiri Reixac, 15-21
Barcelona, Barcelona 08028, ES
P: 0034 93 402 02 40

Recombinant Protein Production Services

In Bioingenium we are experts in the production of recombinant proteins using different expression systems, from bacterial to mammalian cell line expression. We have many years of expertise producing and purifying difficult proteins for many different applications. Our compromise is to fulfill the customer expectations in terms of quantity and also quality of the product.

Bioingenium has developed expressions systems for E. coli and P. pastoris with freedom to operate, so our clients do not have to pay any fees or royalties to third companies. Our aim is to provide the customer with a full service that includes cloning, strain selection, analytics development, upstream development, downstream development and protein characterization.

Expression in P. pastoris

In many cases expression in E. coli could be not adequate for your protein of interests. Pichia pastoris is a good choice for proteins containing di-sulfide bridges, secreted proteins or complex proteins. Our system is able to introduce several genes into the expression strains leaving a wild type genotype without any gene for antibiotics resistance. We are recognized experts in P. pastoris with more than 20 publications in the field.

Expression in E. coli

Our vectors for expression in E. coli allow the production of g per liter quantities of protein. We have a variety of vectors that provides the capacity to use different promoters and chaperones to achieve the highest productivity.

Expression in CHO and HEK

We can provide you with transient expression of your target protein with CHO or HEK cells. High quality and quantity of recombinant proteins can be achieved from cultivation of mammalian cells and a human glycosylation profile is obtained.

Process development

A wide range of services at your disposal: Bioingenium develops an efficient production process for your recombinant proteins

Viability studies are a key point for the success of therapeutic recombinant proteins. A good bioprocess is critical since small errors could be amplified throughout the complete product development. For that reason at Bioingenium we use our experience to select the best expression system for each of the projects in which we are involved. Our processes are thought to be easily scalable to industrial scale and based on biological, technical and economical criteria.

We can cover all the developing steps in the process of production of a recombinant protein from gene design and cloning up to development of the upstream, downstream processes and scale up.

Pichia pastoris freedom to operate system


  • Secretion vs intracellular expression vectors.
  • Multiple promoters and resistance markers.
  • Multi-copy vectors.
  • Strains with chaperone co-expression.
  • For more information contact us at
    Secretion and intracellular expression
    Pichia pastoris is usually a good candidate for secreted proteins thanks to its high secretory capacity. However, also intracellular proteins are produced at high amounts and are recovered after a cell homogenization procedure. Considering the protein original organism and cellular localization we can suggest you different strategies.
    Multiple promoters and resistance markers
    Inducible promoter AOX1 is the first choice for many proteins, nevertheless for certain applications and processes the constitutive promoter GAP is a very good alternative. We can use both and compare the performance expressing your protein of interest.
    Different resistance markers might be selected from a collection and combine them in the most adequate way if you want to express multiple proteins in the same cell.
    Also, our vectors are ready to remove the resistance marker gene once the expression cassette is inserted in the Pichia pastoris genome. You would get a Pichia strain without any antibiotic resistance gene and expressing your protein.
    Multi-copy vectors
    Increase your productivity by increasing the expression cassette copy number in the genome. Ask how and we would help you to improve your protein expression using our vector ready-for-multi-copy expression.

    Strains with chaperone co-expression
    Co-expression of chaperones and regulatory proteins can increase up to 10-fold your protein expression levels.
  • Our customers are biotech companies and pharma companies looking for a small production batch of proteins for proof of concept studies and also companies that are in the process development of the validated product.