Off-target effects are cuts introduced by Cas enzyme in DNA locations other than the specific target. They are produced when the guide binds to other parts of the DNA, different from the target sequence. This generates the introduction of other mutations than those expected.
To avoid this type of effects, there are online tools based on algorithms. In the following post on ABYNTEK BIOPHARMA blog, several tips to avoid these undesired effects are described.
View the full article here: https://www.abyntek.com/how-to-avoid-off-target-events-in-crispr-experiments/?lang=en
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