The CRISPR system has revolutionized the field of genetic engineering. It allows scientist to alter genes in a simple way. The basic principle of CRISPR is based on cutting at a specific point the target DNA, using a nicase enzyme (Cas) which is guided by a fragment of RNA called guide (gRNA). This guide is responsible for directing the enzyme to the point where it should cut. Although it is based on well-known and described cloning techniques, it can be overwhelming to choose components efficiently.
In this article at Abyntek Biopharma blog we talk about some of the key aspects for choosing the RNA guides, which direct the Cas enzyme to the DNA cutting site.
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