Due to the random nature of transgene insertion in the majority of higher eukaryotes, transgenic DNA may integrate into regions of the genome that are transcriptionally repressed, which can result in many cases in transgene silencing. Additionally, transgenes may be incorporated near endogenous regulatory elements, such as transcriptional enhancers or repressors, which can cause their miss-expression

Flanking transgenes with "genetic insulators", DNA sequences that create independent domains of gene expression, can help stabilize them.

We have studied the effect of four different "insulator" sequences over transgene expression levels and variability, and over transgene integration, using NGS. Our results compare the benefits obtained and suggest that different sequences should be used depending on the specific needs of each biotechnology project.

Original Paper:

Pérez-González, A., Caro, E. 2019. Benefits of using genomic insulators flanking transgenes to increase expression and avoid positional effects. Scientific Reports 9, 8474. DOI: 10.1038/s41598-019-44836-6

Subscribe to Directory
Write an Article

Recent News

El diagnóstico genético neonatal mejor...

Un estudio con datos de los últimos 35 años, ind...

Más de 1.500 cambios epigenéticos en e...

Un equipo de investigadores de la Universidad Juli...

Tuneable reverse photochromes in the sol...

A new technique allows the design of solid materia...

Highlight

Eosinófilos. ¿Qué significa tener val...

by Labo'Life

​En nuestro post hablamos sobre este interesante tipo de célula del...

Un estudio de INCLIVA muestra el efecto ...

by INCLIVA

Han desarrollado un estudio para evaluar la correlación entre el teji...

Photos Stream